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Detection of the HSPA2 gene transcription start site by RLM-RACE method. (A) Scheme of procedure using GeneRacerTM Kit (Invitrogen) described in more detail in Materials and Methods. (B) RLM-RACE products obtained after second round of PCR amplification and separated in 2% agarose gel. Legend: T, RLM-RACE reaction with testicular RNA as template; C, control RLM-RACE reaction without template. Numbers on the left-hand side of photograph indicate the size (in bp) of DNA marker. (C) Southern hybridization of RLM-RACE products (from panel B) with HSPA2-specific T2 oligonucleotide probe (for its nucleotide sequence and position in HSPA2 gene see Table 1). (D) Scheme showing structure of the HSPA2 gene promoter region and localization of the main transcription start site marked T(-109). The position of the T(-109) start site was identified by determining the nucleotide sequence of RLM-RACE product cloned into pCR-Blunt vector (Invitrogen). . JTB FlexReport JTB World. In the Community Overview. With MOBILedit Forensic you gain access to these sources and get the full report of all people connected with the suspect. Stay Updated About Outages. A roport de Nice navette bus Cannes ou Monaco avec Nice. Natural Gas Conversion Process. Address Field in Contact Us in Devices. Eight Port 6Gb s PCI Express SATA and SAS RAID Controller PCI Express 2 0 specification and provide Online RAID Level Migration (RLM) Visit the LSI download Center for the most complete list of supported operating systems. Read all about how we work to support and inform you.
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Detection of the HSPA2 gene transcription start site by RLM-RACE method. (A) Scheme of procedure using GeneRacerTM Kit (Invitrogen) described in more detail in Materials and Methods. (B) RLM-RACE products obtained after second round of PCR amplification and separated in 2% agarose gel. Legend: T, RLM-RACE reaction with testicular RNA as template; C, control RLM-RACE reaction without template. Numbers on the left-hand side of photograph indicate the size (in bp) of DNA marker. (C) Southern hybridization of RLM-RACE products (from panel B) with HSPA2-specific T2 oligonucleotide probe (for its nucleotide sequence and position in HSPA2 gene see Table 1). (D) Scheme showing structure of the HSPA2 gene promoter region and localization of the main transcription start site marked T(-109). The position of the T(-109) start site was identified by determining the nucleotide sequence of RLM-RACE product cloned into pCR-Blunt vector (Invitrogen).
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